Recombinant MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase

Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase) . It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13126

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MTAP/3137R

Type

Recombinant

Applications

ELISA, WB, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MTAP antibody is available for research use only.

Storage Conditions

Store the recombinant MTAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human kidney with recombinant MTAP antibody. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.