Phospho-c-Jun Antibody (pT91/93)

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P05412

Host

Mouse

Reactivity

Human

Immunogen

The amino acids surrounding phosphorylated T91 and T93 were used as the immunogen for the phospho-c-Jun antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C-J 4C4/1

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the phospho-c-Jun antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This phospho-c-Jun antibody is available for research use only.

Storage Conditions

Store the phospho-c-Jun antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear

Image Legend

IHC testing of FFPE human cervical tumor with phospho-c-Jun antibody (clone C-J 4C4/1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.