Recombinant IgM Heavy Chain Antibody

This antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein was used as the immunogen for this recombinant IgM Heavy Chain antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RIM373

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant IgM Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant IgM Heavy Chain antibody is available for research use only.

Storage Conditions

Store the recombinant IgM Heavy Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasm, cell surface and secreted

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant IgM Heavy Chain antibody (clone rIM373) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.