HSP27 Antibody

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P04792

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the HSP27 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CPTC-HSPB1-2

Applications

FACS, IF, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HSP27 antibody is available for research use only.

Storage Conditions

Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Predominantly cytoplasmic with some nuclear

Image Legend

IHC staining of FFPE human prostate tissue with HSP27 antibody (clone CPTC-HSPB1-2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.