Recombinant HLA-DQ Antibody (MHC II)
Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P01908, P01906, P01920
Host
Rabbit
Reactivity
Human
Immunogen
Full length recombinant human protein was used as the immunogen for the recombinant HLA-DQ antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
HLA-DQA1/2866R
Type
Recombinant
Applications
FACS, IF
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant HLA-DQ antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant HLA-DQ antibody is available for research use only.
Storage Conditions
Store the recombinant HLA-DQ antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant HLA-DQ antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Immunofluorescent staining of human Raji cells with recombinant HLA-DQ antibody (green, clone HLA-DQA1/2866R) and Reddot nuclear stain (red) .
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