Recombinant HLA-DPB1 Antibody (MHC II)
Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA: 2-4 µg/mL for coating (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P04440
Host
Rabbit
Reactivity
Human
Immunogen
Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the recombinant HLA-DPB1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
HLA-DPB1/2862R
Type
Recombinant
Applications
ELISA, FACS, IF
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant HLA-DPB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant HLA-DPB1 antibody is available for research use only.
Storage Conditions
Store the recombinant HLA-DPB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant HLA-DPB1 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Immunofluorescent staining of human Raji cells with recombinant HLA-DPB1 antibody (clone HLA-DPB1/2862R, green) and Reddot nuclear stain (red) .
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