Melanoma Marker Antibody Cocktail

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

Q16655 (Melan-A), P14679 (Tyrosinase), P40967 (gp100)

Host

Mouse

Reactivity

Human

Immunogen

Recombinant MART-1 protein (A103), recombinant tyrosinase protein (T311) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogens for this Melanoma Marker antibody cocktail.

Clonality

Monoclonal

Isotype

IgG1,IgG2a

Clone

A103 + HMB45 + T311

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Melanoma Cell Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Melanoma Marker antibody cocktail is available for research use only.

Storage Conditions

Store the Melanoma Cell Marker antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human melanoma with Melanoma Marker antibody cocktail (A103 + T311 + HMB45) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.