CD40 Ligand Antibody / TRAP / CD154

CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P29965

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 108-261 from the human protein was used as the immunogen for the CD40 Ligand antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CD40LG/2761

Applications

ELISA, FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD40 Ligand antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD40 Ligand antibody is available for research use only.

Storage Conditions

Store the CD40 Ligand antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, secreted

Image Legend

IHC testing of FFPE human spleen with CD40 Ligand antibody (clone CD40LG/2761) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.