Recombinant CD34 Antibody

This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P28906

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

HPCA1/2598R

Type

Recombinant

Applications

FACS, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD34 antibody is available for research use only.

Storage Conditions

Store the recombinant CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.