Recombinant CD6 Antibody

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular 'scavenger receptor cysteine-rich' (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P30203

Host

Rabbit

Reactivity

Human

Immunogen

Human recombinant protein was used as the immunogen for this recombinant CD6 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

C6/2884R

Type

Recombinant

Applications

FACS, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD6 antibody is available for research use only.

Storage Conditions

Store the recombinant CD6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cell surface and Cytoplasmic

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant CD6 antibody (clone C6/2884R) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.