Recombinant B7-H4 Antibody / VTCN1

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q7Z7D3

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human partial protein was used as the immunogen for this recombinant B7-H4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

B7H4/2652R

Type

Recombinant

Applications

ELISA, FACS, IF, IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant B7-H4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant B7-H4 antibody is available for research use only.

Storage Conditions

Store the recombinant B7-H4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant H7-B4 antibody should be determined by the researcher.

Location

Cytoplasm, plasma membrane

Image Legend

IHC testing of FFPE human testicular carcinoma with recombinant B7-H4 antibody (clone B7H4/2652R) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.