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Recombinant S100A4 Antibody / FSP1

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P26447

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

S100A4/2750R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant S100A4 antibody is available for research use only.

Storage Conditions

Store the recombinant S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human placenta with recombinant S100A4 antibody. Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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