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Recombinant S100A4 Antibody / FSP1

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P26447

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

S100A4/2750R

Type

Recombinant

Applications

WB, FACS, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant S100A4 antibody is available for research use only.

Storage Conditions

Store the recombinant S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody. Predicted molecular weight ~12 kDa.

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