Recombinant S100A4 Antibody / FSP1

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P26447

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PS1A4-1R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant S100A4 antibody is available for research use only.

Storage Conditions

Store the recombinant S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human placenta with recombinant S100A4 antibody (clone PS1A4-1R) . Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.