RAD51 Antibody
RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies. RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Western blot: 1-2 µg/mL
UniProt
Q06609
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 1-134 from the human protein was used as the immunogen for this RAD51 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
RAD51/2753
Applications
ELISA, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the RAD51 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This RAD51 antibody is available for research use only.
Storage Conditions
Store the RAD51 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the RAD51 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human K562, HeLa and MCF-7 cell lysate with RAD51 antibody. Expected molecular weight ~37 kDa.
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
ALOXE3, ID (ALOXE3, Epidermis-type lipoxygenase 3) (Azide free) (HRP)
ALOXE3, ID (ALOXE3, Epidermis-type lipoxygenase 3) (Azide free) (HRP)
Vitronectin Receptor Antibody / Integrin alpha V / ITGAV
Vitronectin Receptor Antibody / Integrin alpha V / ITGAV
