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Recombinant MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q13126

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SMTP-1R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MTAP antibody is available for research use only.

Storage Conditions

Store the recombinant MTAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human kidney with recombinant MTAP antibody (clone SMTP-1R) . Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.

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