Recombinant NGFR Antibody / p75NTR

This antibody is specific for a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or p75 Neurotrophin Receptor (p75NTR) . NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR/p75NTR is a reliable marker for desmoplastic and neurotropic melanomas. It is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR/p75NTR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P08138

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 281-421 from the human protein was used as the immunogen for the recombinant NGFR antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

NGFR/2550R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant NGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant NGFR antibody is available for research use only.

Storage Conditions

Store the recombinant NGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface and cytoplasmic

Image Legend

IHC testing of FFPE human melanoma tissue with recombinant NGFR antibody. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.