Recombinant MUC2 Antibody

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2) . This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro) . Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)

UniProt

Q02817

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant MUC2 antibody. Its epitope has been defined as GTQTP.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MLP/2970R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant MUC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MUC2 antibody is available for research use only.

Storage Conditions

Store the recombinant MUC2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic and cell surface

Image Legend

IHC staining of FFPE human colon tissue with recombinant MUC2 antibody (clone MLP/2970R) . HIER: boil tissue sections in 10mM Tris-HCl buffer, pH 10, for 10-20 min and allow to cool before testing.