Recombinant AURKB Antibody / Aurora B

Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

IHC (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

Q96GD4

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 89-251 was used as the immunogen for this recombinant AURKB antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ARRK2-2R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant AURKB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant AURKB antibody is available for research use only.

Storage Conditions

Store the recombinant AURKB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant AURKB antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human colon tissue with recombinant AURKB antibody (clone ARRK2-2R) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.