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KRT20 Antibody / Cytokeratin 20 / CK20

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. KRT20 (Cytokeratin 20, CK20) is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P35900

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 196-323 from the human protein was used as the immunogen for this KRT20 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CTKN20-1

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the KRT20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This KRT20 antibody is available for research use only.

Storage Conditions

Store the KRT20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

Western blot testing of human HT29 cell lysate with KRT20 antibody (clone CTKN20-1) . Predicted molecular weight ~46 kDa.

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