Recombinant Histone H1 Antibody / Rabbit Monoclonal

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P07305

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

HH1/1784R

Type

Recombinant

Applications

FACS, IF, IHC-P, WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Histone H1 antibody is available for research use only.

Storage Conditions

Store the recombinant Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Nuclear

Image Legend

IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.