Recombinant NKX2.2 Antibody / Rabbit Monoclonal

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

Host

Rabbit

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

NX2/1422R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant NKX2.2 antibody is available for research use only.

Storage Conditions

Store the recombinant NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the recombinant NKX2.2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Nuclear

Image Legend

IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone NX2/1422R) . HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.