Recombinant Ep-CAM Antibody / Rabbit Monoclonal

Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P16422

Host

Rabbit

Reactivity

Human

Immunogen

Human protein was used as the immunogen for the recombinant Ep-CAM antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

EGP40/1556R

Type

Recombinant

Applications

FACS, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Ep-CAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Ep-CAM antibody is available for research use only.

Storage Conditions

Store the recombinant Ep-CAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the recombinant Ep-CAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human colon carcinoma and recombinant Ep-CAM antibody (clone EGP40/1556R) . Required HIER: steam sections in pH6 citrate buffer for 10-20 min.