Recombinant EpCAM Antibody / Rabbit Monoclonal
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL
UniProt
P16422
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant full length protein was used as the immunogen for the recombinant EpCAM antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
EGP40/1555R
Type
Recombinant
Applications
FACS, IHC-P, IF
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant EpCAM antibody is available for research use only.
Storage Conditions
Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Location
Cell surface, cytoplasmic
Image Legend
IHC testing of FFPE human prostate carcinoma and recombinant EpCAM antibody (clone EGP40/1555R) . HIER: steam sections in pH9 EDTA for 10-20 min.
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