Recombinant Glypican-3 Antibody / Rabbit Monoclonal

Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P51654

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A full length human protein was used as the immunogen for the recombinant Glypican-3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GPC3/1534R

Type

Recombinant

Applications

FACS, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Glypican-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Glypican-3 antibody is available for research use only.

Storage Conditions

Store the recombinant Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of MeOH fixed human HepG2 cells with recombinant Glypican-3 antibody (clone GPC3/1534R, green) and Reddot nuclear stain (red) .