Recombinant Cadherin 16 Antibody / Rabbit Monoclonal

Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that CDH16 (kidney-specific cadherin, Cadherin 16) is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, CDH16 is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. CDH16 has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

O75309

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDH16/1532R

Type

Recombinant

Applications

WB, FACS, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Cadherin 16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Cadherin 16 antibody is available for research use only.

Storage Conditions

Store the recombinant Cadherin 16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface with some cytoplasmic

Image Legend

IHC testing of FFPE human renal cell carcinoma with recombinant Cadherin 16 antibody (clone CDH16/1532R) . Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.