Recombinant CD15 Antibody

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P22083

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

FUT4/1478R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant CD15 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD15 antibody is available for research use only.

Storage Conditions

Store the recombinant CD15 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Optimal dilution of the recombinant CD15 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic and cell surface

Image Legend

IHC testing of FFPE human Hodgkin's lymphoma and recombinant CD15 antibody (clone FUT4/1478R) . Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.