Recombinant CD15 Antibody
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P22083
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
FUT4/1478R
Type
Recombinant
Applications
FACS, IF, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CD15 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD15 antibody is available for research use only.
Storage Conditions
Store the recombinant CD15 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic and cell surface
Image Legend
Immunofluorescent staining of human U937 cells with recombinant CD15 antibody (clone FUT4/1478R, green) and Reddot nuclear stain (red) .
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Frequently Asked Questions
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