Recombinant CD99 Antibody / Rabbit Monoclonal

CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) (5-10 µg/mL for 30 min at Room Temp)

UniProt

P14209

Host

Rabbit

Reactivity

Human

Immunogen

Human protein was used as the immunogen for the recombinant CD99 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MIC2/1495R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD99 antibody is available for research use only.

Storage Conditions

Store the recombinant CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the recombinant CD99 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface

Image Legend

IHC testing of FFPE human Ewing's sarcoma with recombinant CD99 antibody (clone MIC2/1495R) . Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.