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Recombinant CD30 Antibody / Rabbit Monoclonal

CD30 is the receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P28908

Host

Rabbit

Reactivity

Human

Immunogen

A partial human protein was used as the immunogen for the recombinant CD30 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

Ki-1/1747R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD30 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD30 antibody is available for research use only.

Storage Conditions

Store the recombinant CD30 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD30 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human Hodgkin's lymphoma stained with recombinant CD30 antibody (clone Ki-1/1747R) . Required HIER: steam sections in pH9, 1mM EDTA for 10-20 min.

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