Recombinant Calponin Antibody / Rabbit Monoclonal

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P51911

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CNN1/1408R

Type

Recombinant

Applications

FACS, IF, IHC-P, WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Calponin antibody is available for research use only.

Storage Conditions

Store the recombinant Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R) . Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.