TOP2A Antibody / Topoisomerase II alpha
This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL (1)
UniProt
P11388
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this TOP2A antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
TPM2A-1
Applications
WB, IF, IHC
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TOP2A antibody is available for research use only.
Storage Conditions
Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC testing of FFPE human tonsil with TOP2A antibody (clone TPM2A-1) .
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