Recombinant PD1 Antibody / Rabbit Monoclonal

PDCD1 (Programmed cell death-1 protein), also called PD1 and CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD1 is expressed by few B-cells, so may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q15116

Host

Rabbit

Reactivity

Human

Immunogen

A partial length human protein was used as the immunogen for the recombinant PD1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PDCD1/1410R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant PD1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant PD1 antibody is available for research use only.

Storage Conditions

Store the recombinant PD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titering of the recombinant PD1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface & cytoplasmic

Image Legend

IHC testing of FFPE human tonsil with recombinant PD1 antibody (clone PDCD1/1410R) . Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.