Recombinant CD31 Antibody / Rabbit Monoclonal

CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16284

Host

Rabbit

Reactivity

Human

Immunogen

Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

C31/1395R

Type

Recombinant

Applications

WB, FACS, IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD31 antibody is available for research use only.

Storage Conditions

Store the recombinant CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titering of the recombinant CD31 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface and cytoplasmic

Image Legend

IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.