Anti-EpCAM Antibody (Extracellular domain)

EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Human partial recombinant protein (extracellular portion) was used as the immunogen for this anti-EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

EPM17-2

Applications

WB, IHC-P, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This anti-EpCAM antibody is available for research use only.

Storage Conditions

Store the anti-EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-EpCAM antibody to be titered up or down for optimal performance.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2) . Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.