Recombinant CD20 Antibody / Rabbit Monoclonal
CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells. [Wiki]
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P11836
Host
Rabbit
Reactivity
Human
Immunogen
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
RMC20-1
Type
Recombinant
Applications
IHC-P, FACS
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This recombinant CD20 antibody is available for research use only.
Storage Conditions
Recombinant CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Predominantly cell surface with some cytoplasmic
Image Legend
IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.
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