Recombinant CD20 Antibody / Rabbit Monoclonal

CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P11836

Host

Rabbit

Reactivity

Human

Immunogen

Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

IGEL/1497R

Type

Recombinant

Applications

FACS, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This recombinant CD20 antibody is available for research use only.

Storage Conditions

Recombinant CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the recombinant CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.