Topoisomerase II alpha Antibody / TOP2A

This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL (1)

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this Topoisomerase II alpha antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TPM2A-2

Applications

WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Topoisomerase II alpha antibody is available for research use only.

Storage Conditions

Store the Topoisomerase II alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.