Recombinant CD1a Antibody / Rabbit Monoclonal

CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein expressed in association with Beta-2 microglobulin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Rabbit

Reactivity

Human

Immunogen

Full length human CD1a protein was used as the immunogen for this recombinant CD1a antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

C1A/1506R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the recombinant CD1a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD1a antibody is available for research use only.

Storage Conditions

Store the recombinant CD1a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD1a antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human skin with recombinant CD1a antibody (clone C1A/1506R) . Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min.