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E-Cadherin Antibody / CDH1

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 2-4 µg/mL

UniProt

P12830

Host

Mouse

Reactivity

Human

Immunogen

A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ECD1-2

Applications

IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This E-Cadherin antibody is available for research use only.

Storage Conditions

Store the E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (clone ECD1-2, green) and Reddot nuclear stain (red) .

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