E-Cadherin Antibody / CDH1
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 2-4 µg/mL
UniProt
P12830
Host
Mouse
Reactivity
Human
Immunogen
A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ECD1-2
Applications
IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This E-Cadherin antibody is available for research use only.
Storage Conditions
Store the E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the E-Cadherin antibody should be determined by the researcher.
Location
Cytoplasmic, membranous
Image Legend
Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (clone ECD1-2, green) and Reddot nuclear stain (red) .
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