Recombinant NKX2.2 Antibody / Rabbit Monoclonal

NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity) . [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Rabbit

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

RMNK2-1

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the recombinant NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant NKX2.2 antibody is available for research use only.

Storage Conditions

Store the recombinant NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titering of the recombinant NKX2.2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Nuclear

Image Legend

IHC testing of FFPE human pancreas with recombinant NKX2.2 antibody (clone RMNK2-1) . HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.