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Recombinant TNF-alpha Antibody / Rabbit Monoclonal

The primary role of TNF is in the regulation of immune cells. TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD) . [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P01375

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant TNF-alpha antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

RMTNFA-1

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant TNF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant TNF-alpha antibody is available for research use only.

Storage Conditions

Store the recombinant TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Optimal dilution of the recombinant TNF-alpha antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic and extracellular (secreted)

Image Legend

IHC staining of FFPE human histiocytoma with recombinant TNF alpha antibody (clone RMTNFA-1) . Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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