Recombinant PD-1 Antibody / Rabbit Monoclonal

Programmed cell death protein 1 is an inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

Q15116

Host

Rabbit

Reactivity

Human

Immunogen

A full length recombinant human protein was used as the immunogen for the recombinant PD-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

RMPD1-1

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant PD-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant PD-1 antibody is available for research use only.

Storage Conditions

Store the recombinant PD-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the recombinant PD-1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface & cytoplasmic

Image Legend

IHC testing of FFPE human tonsil with recombinant PD-1 antibody (clone RMPD1-1) . HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.