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CD74 Antibody

CD74 associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry: 1-2 µg/mL, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P04233

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the CD74 antibody. The epitope is known to be extracellular.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDLA74-1

Applications

FACS, IF, IHC

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD74 antibody is available for research use only.

Storage Conditions

Store the CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface and paranuclear globular

Image Legend

Flow cytometry testing of human Raji cells with CD74 antibody (clone CDLA74-1) ; Red=isotype control, Blue= CD74 antibody.

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