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Muscle Actin Antibody (pan)

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P62736, P68032, P68133

Host

Mouse

Reactivity

Human, Rat

Immunogen

An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PMAC1-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Muscle Actin antibody is available for research use only.

Storage Conditions

Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human leiomyosarcoma with Muscle Actin antibody. FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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