Napsin A Antibody

Napsin-A is a protein that in humans is encoded by the NAPSA gene. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pro-Napsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

O96009

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 189-299 from the human protein were used as the immunogen for the Napsin A antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NPSNA-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Napsin A antibody is available for research use only.

Storage Conditions

Store the Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

Western blot of 1) K562, 2) 293 and 3) A549 cell lysates using Napsin A antibody (clone NPSNA-1) . Expected molecular weight: 38-45 kDa depending on glycosylation level.