VEGF Antibody / VEGFA

Vascular Endothelial Growth Factor is a member of the PDGF/VEGF growth factor family. It is a heparin-binding protein which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 4-8 µg/mL for 30 min at RT (1)

UniProt

P15692

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant protein was used as the immunogen for the VEGF antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VGFA-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This VEGF antibody is available for research use only.

Storage Conditions

Store the VEGF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the VEGF antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.