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GRP94 Antibody / HSP90B1

Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistology (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P14625

Host

Rat

Reactivity

Human

Immunogen

Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SRPR90b

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GRP94 antibody is available for research use only.

Storage Conditions

Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic and nuclear

Image Legend

IHC testing of FFPE human breast carcinoma with GRP94 antibody (clone SRPR90b) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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