CDK1 Antibody / CDC2
Cyclin-Dependent Kinase 1 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. [RefSeq]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
UniProt
P06493
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the CDK1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CDVC2-1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CDK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CDK1 antibody is available for research use only.
Storage Conditions
Store the CDK1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/cdk1-antibody-cdc2-cdvc2-1-v7171
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the CDK1 antibody may be required for optimal performance.
Location
Nuclear and cytoplasmic
Image Legend
Western blot testing of human HeLa cell lysate with CDK1 antibody (clone CDVC2-1) . Predicted molecular weight ~33 kDa.
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